Are you in the Right Flight??

Dexter- Has got his Blood Splatter Analysis done! What About You??…. Yes Guys! This is where blood takes flight and we as forensic scientist need to know the Direction of Flight- i.e The trajectory of a blood drop which can be established by its angle of impact and directionality angle.

Now you need to know the basics in Direction in Blood Splatter. Here are few terminologies:

Draw-Back Effect – Blood in the barrel of a firearm that has been drawn backward into the muzzle.

Drip Pattern — A bloodstain pattern which results from blood dripping into blood.

Expirated Blood – Blood that is blown out of the nose, mouth, or a wound as a result of air pressure and/or air flow which is the propelling force.

Flight Path – The path of the blood drop, as it moves through space, from the impact site to the target.

Flow Pattern – A change in the shape and direction of a bloodstain due to the influence of gravity or movement of the object.

Forward Spatter – Blood which travels in the same direction as the source of energy or force which caused the spatter.

High Velocity Impact Spatter (HVIS) — A bloodstain pattern caused by a high velocity impact /force to a blood source such as that produced by gunshot or high speed machinery.

Impact Pattern – Bloodstain pattern created when blood receives a blow or force resulting in the random dispersion of smaller drips of blood.

Impact Site — That point where force encounters a source of blood.

Low Velocity Impact Spatter (LVIS) – A bloodstain pattern that is caused by a low velocity impact/force to a blood source.

Medium Velocity Impact Spatter (MVIS) – A bloodstain pattern caused by a medium velocity impact/force to a blood source. A beating typically causes this type of spatter.

Passive Drop (Bleeding) – Bloodstain drop(s) created or formed by the force of gravity acting along.

Point (Area) of Convergence – The common point (area), on a two dimensional surface, over which the directionality of several blood drops can be retraced.

Point (Area) of Origin — The common point (area) in a three dimensional space to which the trajectories of several blood drops can be retraced.

Projected Blood Pattern — A bloodstain pattern that is produced by blood released under pressure as opposed to an impact, such as arterial spurting

Angle of Impact: The steeper the impact, the more elliptical or elongated, the blood drop

For a complete list of bloodstain pattern terms, as recommended by the International Association of
Blood Stain Pattern Analysts. (IABPA), go to: http://www.iabpa.org/
These terms should serve as a guide, for those who work and teach in the field of Blood Stain Pattern Analysis

FORENSIC DNA

DNA typing, since it was introduced in the mid-1980s, has revolutionized forensic science and the ability of law enforcement to match perpetrators with crime scenes. Thousands of cases have been closed and innocent suspects freed with guilty ones punished because of the power of a silent biological witness at the crime scene.
DNA technologies used in forensic investigations

Restriction Fragment Length Polymorphism (RFLP)
RFLP is a technique for analyzing the variable lengths of DNA fragments that result from digesting a DNA sample with a special kind of enzyme. RFLP was one of the first applications of DNA analysis to forensic investigation.

PCR Analysis
Polymerase chain reaction (PCR) is used to make millions of exact copies of DNA from a biological sample. DNA amplification with PCR allows DNA analysis on biological samples as small as a few skin cells.

STR Analysis
Short tandem repeat (STR) technology is used to evaluate specific regions (loci) within nuclear DNA. Variability in STR regions can be used to distinguish one DNA profile from another.

Mitochondrial DNA Analysis
Mitochondrial DNA analysis (mtDNA) can be used to examine the DNA from samples that cannot be analyzed by RFLP or STR.

Y-Chromosome Analysis
The Y chromosome is passed directly from father to son, so analysis of genetic markers on the Y chromosome is especially useful for tracing relationships among males or for analyzing biological evidence involving multiple male contributors.

FORENSIC BALLISTIC COURSE DETAILS

Forensic Arson Analysis

When detectives arrive at the scene of a fire, the first thing that they do is interview any witnesses to the fire, for example, the person who called the fire brigade and those who arrived before the fire department, whom may have seen how the fire began. When the fire has been controlled, the temperature falls, allowing the firefighters to make the building safe for investigation.  Similar to burglary and theft, arson is also a crime of stealth. The perpetrator of arson does not want to be seen committing his cowardly act. Most arson fires, therefore, occur at night and normally when no one is in the structure. Arson-for-profit is usually planned well ahead and the insured usually has a solid alibi far from the scene. 

 The investigation into how the fire started involves beginning at the lower levels, as fire travels upwards. The signs investigators search for when looking for the place the fire may have started include lingering heat, how deep the charring is, the flaking of building materials like cement and plaster, distorted plastic, metal and glass resulting from prolonged burning, damaged ceiling and structural damage.Samples are sealed in airtight containers and then tested for residues of accelerant liquid that might have been used to start the fire. These are the most common tests performed by forensics labs during an arson investigation:

• Static headspace heats the sample, causing the residue to separate out and vaporize into the top, or "headspace" of the container. That residue is then injected into a gas chromatograph, where it's broken apart to analyze its chemical structure.
• Passive headspace heats the sample and the residue collects onto a carbon strip in the container. Then the residue collected is injected into a gas chomatograph/mass spectrometer for analysis.
• Dynamic headspace bubbles liquid nitrogen gas through the sample and captures the residue onto an absorbent trap. The trapped compounds are then analyzed using gas chromatography.

References:

1. How Stuff Works
2. Fire Cops: On the Case with America's Arson Investigators
3. Fire Investigator Field Guide
4. Thinkquest team

Forensic Saliva Analysis

Body fluids, whether excreted or secreted, help our forensic experts compile detailed reports on sexual assaults, death, and the identification of attackers among other things.A forensic investigation can involve the analysis of body fluids, including saliva, for evidence of toxins and both prescription and illicit drugs.Saliva can be of forensic significance because traces of drugs that are circulating in the body can be present in saliva. The composition of the saliva accurately mirrors the proteins that are present in both the blood and the urine. Thus, testing of saliva, which is easier and less obtrusive than obtaining a blood or urine sample, can be used to reveal the presence of prescription and illicit drugs.

 Saliva is presented as an alternative matrix in the establishment of drug abuse. The ultimate salivary concentration is determined by the route of administration, the salivary pH, the degree of plasma protein binding,and the physico-chemical properties of the abused drug. Since the saliva/plasma ratio can exceed 1, saliva might be a better analytical tool than blood during roadside testing of potentially intoxicated drivers

 Virkler and Lednev add that Raman spectroscopy has great potential as just such a non-destructive tool. In 2008, the team reported that it could be used to identify bodily fluids at a crime scene but those experiments were carried out with just a single sample of each type of fluid. Now, the team has extended the work significantly to investigate the potential for spectroscopic differences among different "donors" of the same fluid.
The researchers have used near-infrared (NIR) Raman spectroscopy to obtain spectra for pure dried human saliva samples from several donors in a controlled laboratory environment. By applying principal component analysis (PCA) on the spectra they demonstrated that dry saliva is a particularly heterogeneous substance. However, the Raman spectra can be described as being a linear combination of a fluorescent background and three spectroscopic components.

New Forensics Tool Can Determine a Person's Age from a Spit Sample

Researchers at UCLA have figured out how to determine age to within five years from nothing more than a saliva sample.

The method relies on a process called methylation, which is a chemical change to one of the four building blocks of a person’s DNA. Methylation changes as our bodies grow older, contributing to age related diseases. In extracting DNA from saliva samples from more than 100 test subjects, the team found that it could zero in on a person’s age within five years by looking at just two of the 3 billion blocks that make up the human genome--such is the strong correlation between methylation and age.

 

References:

1. Enotes
2. Sourcebook in forensic serology, immunology, and biochemistry
3. Scientific Protocols for Forensic Examination of Clothing (Protocols in Forensic Science)
4. Forensic Biology: Identification and DNA Analysis of Biological Evidence

Saliva Can Reveal Person's Age

Geneticists of University of California, Los Angeles, have developed a new saliva test which can accurately predict a person's age. 

A newly patented test based on the research, for example, could offer crime-scene investigators a new forensic tool for pinpointing a suspect's age.

"Our approach supplies one answer to the enduring quest for reliable markers of aging," said principal investigator Dr. Eric Vilain, a professor of human genetics, pediatrics and urology at the David Geffen School of Medicine at UCLA.

"With just a saliva sample, we can accurately predict a person's age without knowing anything else about them," he added.

Vilain and his colleagues looked at a process called methylation - a chemical modification of one of the four building blocks that make up our DNA.

"While genes partly shape how our body ages, environmental influences also can change our DNA as we age," explained Vilain.

"Methylation patterns shift as we grow older and contribute to aging-related disease," he added.

"Methylation's relationship with age is so strong that we can identify how old someone is by examining just two of the 3 billion building blocks that make up our genome," said first author Sven Bocklandt, a former UCLA geneticist now at Bioline.

The study has been published in the online edition of the Public Library of Science (PLoS) ONE

Source-ANI

Forensic Human identification

People can be identified by their fingerprints. This assertion is supported by the philosophy of friction ridge identification, which states that "Friction ridge identification is established through the agreement of friction ridge formations, in sequence, having sufficient uniqueness to individualize".

Friction ridge identification is also governed by four premises or statements of fact:

   1. Friction ridges develop on the fetus in their definitive form prior to birth.
   2. Friction ridges are persistent throughout life except for permanent scarring, disease or decomposition after death.
   3. Friction ridge paths and the details in small areas of friction ridges are unique and never repeated.
   4. Overall, friction ridge patterns vary within limits which allow for classification.

People can also be identified from traces of their DNA by DNA fingerprinting, from their teeth or bite by forensic odontology, from a photograph or a video recording by facial recognition systems, from the video recording of their walk by gait analysis, from an audio recording by voice analysis, from their handwriting by handwriting analysis, from the content of their writings by their writing style (e.g. typical phrases, factual bias, and/or misspellings of words), or from other traces using other biometric techniques.

Body identification is a subfield of forensics concerned with identify someone from their remains.

Body identification is a subfield of forensic science wherein investigators need to identify a body. Forensic (literally, "for the courts") purposes are served by rigorous scientific forensic identification techniques, but these are generally preceded by simply asking bystanders or other persons for the victim's name.

If a body is not badly decomposed or damaged, two persons (or one) who knew the deceased well should visually confirm the identity.

Authorities will also compare supportive documents such as driver's license, passport, or other authoritative photo ID before accepting a personal identification with which to further their investigative and/or forensic purposes.

Of course, any formal investigation should "reality check" additional forensic and scientific evidence to reinforce or question the supposed identity of the victim. Reliable identification becomes increasingly difficult as time passes.

 SOURCE : WIKIPEDIA

 IMPORTANT LINKS:

Forensic Human Identification Course

Forensic Human Identification: An Introduction

Recovery, Analysis, and Identification of Commingled Human Remains

Human and Nonhuman Bone Identification: A Concise Field Guide