Forensic Saliva Analysis

Body fluids, whether excreted or secreted, help our forensic experts compile detailed reports on sexual assaults, death, and the identification of attackers among other things.A forensic investigation can involve the analysis of body fluids, including saliva, for evidence of toxins and both prescription and illicit drugs.Saliva can be of forensic significance because traces of drugs that are circulating in the body can be present in saliva. The composition of the saliva accurately mirrors the proteins that are present in both the blood and the urine. Thus, testing of saliva, which is easier and less obtrusive than obtaining a blood or urine sample, can be used to reveal the presence of prescription and illicit drugs.

Saliva is presented as an alternative matrix in the establishment of drug abuse. The ultimate salivary concentration is determined by the route of administration, the salivary pH, the degree of plasma protein binding,and the physico-chemical properties of the abused drug. Since the saliva/plasma ratio can exceed 1, saliva might be a better analytical tool than blood during roadside testing of potentially intoxicated drivers

Virkler and Lednev add that Raman spectroscopy has great potential as just such a non-destructive tool. In 2008, the team reported that it could be used to identify bodily fluids at a crime scene but those experiments were carried out with just a single sample of each type of fluid. Now, the team has extended the work significantly to investigate the potential for spectroscopic differences among different “donors” of the same fluid.

The researchers have used near-infrared (NIR) Raman spectroscopy to obtain spectra for pure dried human saliva samples from several donors in a controlled laboratory environment. By applying principal component analysis (PCA) on the spectra they demonstrated that dry saliva is a particularly heterogeneous substance. However, the Raman spectra can be described as being a linear combination of a fluorescent background and three spectroscopic components.

Forensic Preliminary Testing for Saliva (amylase)

Phadebas tablet method

1. Place 50 ul of each described dilution from above into a

test tube.

2. Add 1.0 ml of water, and ¼ Phadebas tablet (cut with scalpel

or razor blade CAREFULLY). Add the ¼ tablet using

forceps, NOT fingers to handle/cut the tablet.

3. Vortex to mix thoroughly.

4. Incubate at 37C for 30 minutes.

5. Add .25 ml of 0.5 M Sodium Hydroxide to each tube to stop

the reaction.

6. Centrifuge for 5 minutes.

7. A transparent dark blue supernatant

LATEST!!- New Forensics Tool Can Determine a Person’s Age from a Spit Sample

Researchers at UCLA have figured out how to determine age to within five years from nothing more than a saliva sample. The method relies on a process called methylation, which is a chemical change to one of the four building blocks of a person’s DNA. Methylation changes as our bodies grow older, contributing to age related diseases. In extracting DNA from saliva samples from more than 100 test subjects, the team found that it could zero in on a person’s age within five years by looking at just two of the 3 billion blocks that make up the human genome–such is the strong correlation between methylation and age.

References:

1. Enotes

2. Sourcebook in forensic serology, immunology, and biochemistry

3. Scientific Protocols for Forensic Examination of Clothing (Protocols in Forensic Science)

4. Forensic Biology: Identification and DNA Analysis of Biological Evidence